Chemical constituents from the roots of Homonoia riparia / 药学学报

A new compound and twelve known compounds were isolated from the ethyl acetate extract of the roots of Homonoia riparia Lour, which are used in folk medicine for treatment of hepatitis, bellyache and scald, by the method of silica gel column chromatography repeatedly with a gradient of PE-EtOAc, PE-Me2CO, CHCl3-Me2CO, CHCl3-MeOH. Their structures were identified as a new compound 1-oxo-aleuritolic acid (1), and twelve known compounds aleuritolic acid (2), 3-acetoxy-aleuritolic acid (3), taraxerone (4), taraxerol (5), methyl 3-acetoxy-12-oleanen-28-oate (6), 3-acetoxy-12-oleanen-28-ol (7), ursolic acid (8), lupenol (9), 3β-acetoxy-lupenol (10), cleomiscosin A (11), chrysophanol (12), and gallic acid (13), which were obtained from this plant for the first time, by the spectroscopic techniques of NMR, HMBC, IR and MS, separately. Among the cytotoxicities evaluation of compounds 1-3 towards AGZY 83-a (human lung cancer cells) and SMMC-7721 (human liver cancer cells) tumor cells was assayed by MTT methods with cis-dichlorodiamminoplatinum (DDP) used as positive control. Compound 2 exerted weak activity against AGZY 83-a with the IC50 value of 33.055 μg·mL-1, while 1 and 3 showed no activity to these two cell lines.

Effect of extract from Tripterygium hypoglaucum on proliferation and apoptosis of vascular smooth muscle cells / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effect of THW-4, an extract from Trypterygium hypoglaucum on proliferation and apoptosis of vascular smooth muscle cells (VSMC).</p><p><b>METHODS</b>VSMC derived from rabbit aorta were cultured in vitro and different concentrations of THW-4 were added in experimental groups. Cell proliferation was evaluated by MTT and apoptosis by transmission electron microscopy (TEM), TUNEL assay and Annexin V-FITC/PI double labelled assay.</p><p><b>RESULTS</b>The inhibitory effects of THW-4 on proliferation of VSMC displayed dose-time dependently, with the IC50 value of 15.6 microg/L at 48 hrs. Incubated with THW-4 (10-100 microg/L) for 56 hrs, VSMC mainly appeared early stage apoptosis and the percentage of apoptosis was found to raise along with the increase of the THW-4 concentration. Typical images of apoptosis could be observed under TEM and TUNEL assay showed increase of DNA segments with karyorrhexis and pyknosis after THW-4 treatment for 72 hrs. Analysis of cell cycle indicated the THW-4 mainly lead to the blockage of VSMC in G2/M stage.</p><p><b>CONCLUSION</b>THW-4 could inhibit the proliferation and induce the apoptosis of VSMC in vitro, suggesting that THW-4 is a potential agent for prevention of restenosis following angioplasty.</p>

Studies on chemical constituents of Toona sinensis / 中草药

Object To proceed our continual search of insecticides and potentially useful pharmaceutics in plants of the Meliaceae family Methods Chemical constituents in the ethanolic extract of the leaves of Toona sinensis (A Juss ) Roem were separated and identified Results 6 compounds were isolated and identified as: 6, 7, 8, 2′ tetramethoxy 5, 6′ dihydroxy flavone (Ⅰ); 5, 7 dihydroxy 8 methoxy flavone (Ⅱ); kaempferol (Ⅲ); 3 hydroxy 5, 6 epoxy 7 megastigmen 9 one (Ⅳ), ethyl gallate (Ⅴ) and scopoletin (Ⅵ) by spectral methods Conclusion Compound Ⅳ was obtained from Toona Roem. for the first time